Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: Surface markers and cytokines expressed by naïve and memory T-cells

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Expressing

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: .Stimulated antigen-experienced T-cells gain effector function without cell division. PBMC from healthy adult donors depleted of CD45RA+ T-cells, were stained with 0·25 µm CFSE and were then stimulated for 4 days with PHA or SEB, or for 6 days by MLR. Shown is a dot plot panel of surface markers and cytokine production for antigen-experienced CD4+ or CD8+ gated T-cells.

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: IL-7 drives proliferation of naïve T-cells in MHC independent fashion. Cord blood PBMC were stained with 0·25 µm CFSE and were then cultured for 9 days in 10 ng/ml IL-7 with isotypic control antibodies (a) or 10 µg/ml blocking antibodies recognizing HLA A, B, C, DR, DP, and DQ (b) Dot plots of CD4+ or CD8+ gated T-cells show expression patterns of CD45RA, and CD45RO with respect to cell division. Boxed areas of CD4 and CD8 plots represent the percentage of cultured cells that had proliferated more than three times for IL-7 alone (a) and IL-7+ anti-MHC antibodies (b).

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining, Cell Culture, Control, Blocking Assay, Expressing

Journal:

Article Title: Expansion of activated human na?ve T-cells precedes effector function

doi: 10.1046/j.1365-2249.2002.02015.x

Figure Lengend Snippet: IL-2 is Required for IL-7 induced expansion of memory T-Cells present in umbilical cord blood. Cord blood PBMC were stained with 0·25 µm CFSE and were then cultured for 9 days in 10 ng/ml IL-7 with or without 10 µg/ml depleting antibodies to IL-2. After culturing, PBMC were stained with CD4 and CD45RO or CD45RA, and CD8 and CD45RA or CD45RO. Dot plots of CD4+ or CD8+ T-cells show expression patterns of CD45RA, CD45RO, CD25, and IFN-γ with respect to cell division.

Article Snippet: Antigen-experienced cells were purified by incubating cells with a FITC-conjugated antibody to CD45RA, then depleted by MACS sorting using anti-FITC magnetic microbeads (Miltenyi, Auburn, CA, USA).

Techniques: Staining, Cell Culture, Expressing

Journal: medRxiv

Article Title: Identification and validation of heterotypic cell-in-cell structure as an adverse prognostic predictor for young patients of resectable pancreatic ductal adenocarcinoma

doi: 10.1101/2020.07.08.20148825

Figure Lengend Snippet: ( A ) Unprocessed composite core multiplex stained with E-cadherin, CD68, CD45, and DAPI. ( B ) Unmixed composite core pseudo-colored with yellow for E-cadherin, green for CD68, red for CD45, and blue for DAPI. ( C ) Spectral parameters for image unmixing. Fluorescent signals for different targets were captured based on the spectra indicated. ( D ) Images of single or merged channels displaying the boxed region in ( C ). Scale bar: 20 μm. ( E ) Representative images for the 4 CIC subtypes as indicated; scale bars: 5 μm;

Article Snippet: Samples were first stained with anti-CD45 antibody (mouse mAb from Boster, BM0091) at a dilution of 1:400 using Opal Multiplex tissue staining kit (Perkin Elmer, NEL791001KT) according to the manufacturer’s standard protocol.

Techniques: Multiplex Assay, Staining

Journal: medRxiv

Article Title: Identification and validation of heterotypic cell-in-cell structure as an adverse prognostic predictor for young patients of resectable pancreatic ductal adenocarcinoma

doi: 10.1101/2020.07.08.20148825

Figure Lengend Snippet: (A) CIC structures indicated by arrows in PDAC tissues stained by H&E. Boxed regions are zoomed in at the right. (B) CIC structures in PDAC tissues stained with antibodies for CD68 or CD45, respectively, by IHC. Boxed regions are zoomed in at the bottom. (C) Number of tissues positive in each CIC subtype. (D) The compositions of CIC subtypes in different TNM stages.

Article Snippet: Samples were first stained with anti-CD45 antibody (mouse mAb from Boster, BM0091) at a dilution of 1:400 using Opal Multiplex tissue staining kit (Perkin Elmer, NEL791001KT) according to the manufacturer’s standard protocol.

Techniques: Staining